Oxford University Press' eCAM Journal's article on homeopathic research on cell cultures

[Dana Ullman, MPH]

Dear Friends of Homeopathy,

by Oxford University Press and which has become the most respected
peer-review journal in the field of alternative and complementary medicine.
Of special interest is the fact that this study has repeated shown that
homeopathically potentized doses have dramatic effects on various kinds of
cancer cells, not just in the short-term but the long-term. This research
also shows that various homeopathic medicines have dramatic effects on gene
expression.
Sunila ES, Kuttan R, Preethi KC, Kuttan G. Dynamized Preparations in Cell
Culture. Evid Based Complement Alternat Med. eCAM 2009;6(2)257-263
doi:10.1093/ecam/nem082
http://ecam.oxfordjournals.org/cgi/cont ... 2/257?etoc
ABSTRACT:
models are limited, there are even fewer reports on the in vitro action of
these dynamized preparations. We have evaluated the cytotoxic activity of
30C and 200C potencies of ten dynamized medicines against Dalton's Lymphoma
Ascites, Ehrlich's Ascites Carcinoma, lung fibroblast (L929) and Chinese
Hamster Ovary (CHO) cell lines and compared activity with their mother
tinctures during short-term and long-term cell culture. The effect of
dynamized medicines to induce apoptosis was also evaluated and we studied
how dynamized medicines affected genes expressed during apoptosis. Mother
tinctures as well as some dynamized medicines showed significant
cytotoxicity to cells during short and long-term incubation. Potentiated
alcohol control did not produce any cytotoxicity at concentrations studied.
The dynamized medicines were found to inhibit CHO cell colony formation and
thymidine uptake in L929 cells and those of Thuja, Hydrastis and
Carcinosinum were found to induce apoptosis in DLA cells. Moreover,
dynamized Carcinosinum was found to induce the expression of p53 while
dynamized Thuja produced characteristic laddering pattern in agarose gel
electrophoresis of DNA. These results indicate that dynamized medicines
possess cytotoxic as well as apoptosis-inducing properties.
FROM THE "DISCUSSION SECTION OF THE ARTICLE:
We found that in short-term cytotoxicity research, some of the dynamized
preparations showed significant cytotoxic actions against cancer cell lines
and at times the activity was higher than that of the mother tinctures. For
example, Conium at 200C potency was more cytotoxic than its mother tincture
and that the cytotoxicity induced by Carcinosinum was higher at 200C than at
30C potency indicating that dynamization induces the cytotoxic potential of
these medications. Results were more pronounced during MTT assay in which a
longer period of incubation was involved. Many dynamized preparations at
potency of 200C inhibited the growth of L929 cells. Clonogenic assay using
CHO cells is a standard method to determine growth inhibitory activity of
the drugs and we found that dynamized preparations of Thuja, Hydrastis,
Carcinosinum and Podophyllum at 200C potency almost completely inhibited the
CHO colony formation. As in other cases, Conium 200C was more active than
30C. We have confirmed the cytotoxic potential of dynamized preparations by
thymidine uptake, for the marker of the inhibition DNA synthesis. As in the
case other experiments, DNA synthesis was significantly inhibited by several
dynamized preparations.
Dana Ullman, MPH
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