[H] Horizon TV Program
Posted: Tue Jan 13, 2004 2:45 am
Hello!
This all come to light due to the research done by Dana Ullman.
I just received two letters from him-- one to BBC and one to ABC.
The Horizon Program stated that it was replicating the experiments
conducted my Dr. Madeline Ennis that showed activity of ultra
molecular dilutions.
The reality is that the it has just been discovered that the
experimenter who conducted the research, Wayne Turnbull of Guys
Hospital, never communicated with Professor Ennis to assure that he
was properly conducting the experiment. Thus, the experiment
conducted did NOT replicate her trials in the following ways (as
quoted by Ennis):
* He left the blood to sediment for 4 h - this is far longer than we ever used.
* He used completely different buffers including the addition of
foetal calf serum.
* He added in an ammonium chloride lysis step.
* He added in a passive sensitization step and said that the cells
were used within 24 h for the assay (we would not leave our basophils
hanging around for such a long time). They were left in the presence
of 10% foetal calf serum (this is not a defined medium and could
contain many things that might influence an assay).
* I do not know where he got his anti-IgE from (this was not given in
the protocol) and I did tell him that anti-IgE varies from supplier
to supplier and indeed from batch to batch.
* Speaking to Wayne, he really knows very little about basophils,
although he is an experienced flow cytometrist. He knew nothing about
the commercially available kits looking at basophil activation (there
are 2 good ones on the market) nor about the European study investing
drug allergies using this methodology.
This is, indeed, poor Science. And it is false reporting-- saying
that the experiment HAS been replicated, when it has not been.
Dana is in correspondence with both BBC and ABC about this. Perhaps
those who live in Australia can pass this information on to the the
TV channel down there who aired this documentary. I will certainly do
so in NZ.
JW
_______________________________________________
Homeopathy Mailing List
homeopathy@homeolist.com
http://www.homeolist.com/mailman/listinfo/homeopathy
This all come to light due to the research done by Dana Ullman.
I just received two letters from him-- one to BBC and one to ABC.
The Horizon Program stated that it was replicating the experiments
conducted my Dr. Madeline Ennis that showed activity of ultra
molecular dilutions.
The reality is that the it has just been discovered that the
experimenter who conducted the research, Wayne Turnbull of Guys
Hospital, never communicated with Professor Ennis to assure that he
was properly conducting the experiment. Thus, the experiment
conducted did NOT replicate her trials in the following ways (as
quoted by Ennis):
* He left the blood to sediment for 4 h - this is far longer than we ever used.
* He used completely different buffers including the addition of
foetal calf serum.
* He added in an ammonium chloride lysis step.
* He added in a passive sensitization step and said that the cells
were used within 24 h for the assay (we would not leave our basophils
hanging around for such a long time). They were left in the presence
of 10% foetal calf serum (this is not a defined medium and could
contain many things that might influence an assay).
* I do not know where he got his anti-IgE from (this was not given in
the protocol) and I did tell him that anti-IgE varies from supplier
to supplier and indeed from batch to batch.
* Speaking to Wayne, he really knows very little about basophils,
although he is an experienced flow cytometrist. He knew nothing about
the commercially available kits looking at basophil activation (there
are 2 good ones on the market) nor about the European study investing
drug allergies using this methodology.
This is, indeed, poor Science. And it is false reporting-- saying
that the experiment HAS been replicated, when it has not been.
Dana is in correspondence with both BBC and ABC about this. Perhaps
those who live in Australia can pass this information on to the the
TV channel down there who aired this documentary. I will certainly do
so in NZ.
JW
_______________________________________________
Homeopathy Mailing List
homeopathy@homeolist.com
http://www.homeolist.com/mailman/listinfo/homeopathy